Presence of Specific Growth Hormone Binding Sites in Rainbow Trout (Oncorhynchus Mykiss) Tissues: Characterization of the Hepatic Receptor.
The present work outlines the presence of specific binding for Chinook salmon growth hormone (sGH) in different tissue preparations of rainbow trout. Optimal incubation conditions (pH, Tris, MgCl2) were determined. Specific binding was very sensitive to salt concentration during incubation. The specific binding reached a plateau after 15 and 25 hr of incubation at 12 and 4